Bio Safety Cabinets, commonly referred to as biological safety cabinets or Clean Air Cabinets, play a pivotal role in creating secure laboratory environments. Moreover, they provide critical protection for both the user and the surrounding environment by containing hazardous substances effectively. Explore our complete range of Clean Room Equipment for comprehensive laboratory solutions.
Our products, manufactured to top-class standards and available in customized sizes, are designed by Peter Air Systems according to specific requirements. In addition, these cabinets ensure optimal safety while maintaining flexibility for various laboratory needs.
Furthermore, these cabinets are essential in labs, functioning like special boxes that guarantee safety. As a result, they are crucial for making laboratories safe and secure for conducting experiments and handling hazardous substances efficiently.
The biosafety cabinet working principle is based on three critical layers of protection: personnel protection, product protection, and environmental protection. Understanding this biosafety cabinet principle helps laboratory managers select the right equipment for their applications.
Step 1: Air Intake (Personnel Protection)
Room air is drawn through the front grille at a minimum velocity of 0.38 m/s (75-100 fpm). This creates an air barrier that prevents hazardous aerosols from escaping the cabinet and entering the operator’s breathing zone .
Step 2: HEPA Filtration
The intake air passes through a pre-filter (capturing large particles) and then through a HEPA filter with 99.99% efficiency at 0.3 microns. The HEPA filter uses three mechanisms: impaction, interception, and diffusion to trap particles smaller than the filter pore size .
Step 3: Downward Laminar Airflow (Product Protection)
Filtered air flows vertically (downward) over the work surface, creating ISO Class 5 air quality. This prevents cross-contamination between samples and maintains sterility .
Step 4: Air Split and Recirculation
As air reaches the work surface, it splits – approximately 30-40% is exhausted through the rear grille, while 60-70% is recirculated through the supply HEPA filter back into the work zone .
Step 5: Exhaust (Environmental Protection)
The exhausted air passes through a second HEPA filter before being discharged either back into the laboratory (Type A2) or outside the building via hard ducting (Type B2) .
Airflow Pattern Summary by Class:
| Class/Type | Face Velocity (fpm) | Airflow Pattern | Recirculation % |
|---|---|---|---|
| Class I | 75 | In at front; rear and top through HEPA | 0% |
| Class II Type A2 | 100 | 70% recirculated; 30% exhaust through HEPA | 70% |
| Class II Type B1 | 100 | 30% recirculated; 70% exhaust hard ducted | 30% |
| Class II Type B2 | 100 | No recirculation; 100% exhaust hard ducted | 0% |
| Class III | N/A | Gas-tight enclosure; supply & exhaust via 2 HEPA filters | 100% recirculated |
Source: NSF/ANSI Standard 49 and AS/NZS 2252 standards
Understanding biological safety cabinets types is essential for selecting the right cabinet for your application. The biosafety cabinet classification system (Class I, II, and III) is defined by international standards including NSF/ANSI 49 and EN 12469 .
Protection Level: Personnel and environmental protection only (NO product protection)
Airflow: Room air is drawn in through the front opening at 75 fpm, passes over the work surface, and is exhausted through a HEPA filter.
Best For: Work with low to moderate risk agents where product protection is not required.
Limitation: Unfiltered room air passes over the work surface, so samples can become contaminated.
Protection Level: Personnel, product, AND environmental protection
Airflow: HEPA-filtered air flows downward over the work surface, creating an ISO Class 5 clean zone .
Sub-types within Class II:
| Type | Recirculation | Exhaust Method | Chemical Compatibility |
|---|---|---|---|
| Type A1 | 70% recirculated | Room or canopy connection | No (trace amounts only) |
| Type A2 | 70% recirculated | Room or canopy connection | Trace amounts (if ducted) |
| Type B1 | 30% recirculated | Hard ducted (70% exhaust) | Yes (low levels) |
| Type B2 | 0% recirculated | Hard ducted (100% exhaust) | Yes (full compatibility) |
Class II Type A2 Recommendation: This is the PREFERRED type for most microbiology and clinical laboratories. It offers excellent protection, is energy-efficient, and does not require hard ducting to the outside.
Protection Level: Highest level – personnel, product, and environmental protection for Biosafety Level 4 (BSL-4) agents
Design: Gas-tight enclosure (fully sealed) with glove ports for manipulation. All materials enter and leave through a dunk tank or double-door autoclave .
Airflow: Supply air is HEPA-filtered; exhaust passes through two HEPA filters before leaving the cabinet .
Best For: Work with highly infectious agents (Ebola, smallpox), emerging diseases, and maximum containment requirements .
The biosafety cabinet HEPA filter is the most critical component for contamination control. Understanding how it works helps laboratory managers make informed purchasing decisions.
Many people mistakenly believe that a HEPA filter with 0.3-micron pore size cannot trap particles smaller than 0.3 microns. However, this is incorrect. The HEPA filter is more like a “forest” than a single-layer net .
Three Mechanisms of Particle Capture:
Most Penetrating Particle Size (MPPS): The MPPS for HEPA filters is approximately 0.3 microns – this is actually the hardest particle size to capture! Particles smaller OR larger than 0.3 microns are trapped with even higher efficiency .
| Parameter | Specification |
|---|---|
| Efficiency | 99.999% at 0.3 microns (H14 grade) |
| Pre-filter Efficiency | 95% down to 5 microns |
| Test Standard | EN 1822 / ISO 14644 |
| Filter Housing | SS 304 / Powder coated GI |
| DOP Test Ports | Provided for integrity testing |
The primary biosafety cabinet function is to provide three layers of protection. Understanding the biosafety cabinet purpose helps justify the investment.
1. Personnel Protection (Most Important)
The cabinet protects laboratory workers from exposure to infectious aerosols generated during procedures like pipetting, centrifugation, or vortexing .
2. Product Protection (Sample Protection)
HEPA-filtered laminar airflow prevents airborne contaminants from settling on your samples, cultures, or media . This is essential for cell culture and sterile work.
3. Environmental Protection
Exhaust air is HEPA-filtered before being released back into the laboratory or outside, preventing the spread of biological agents .
| Model | External Size (W x D x H) | Working Size (W x D x H) | Typical Application |
|---|---|---|---|
| BSC-2ft | 750 x 750 x 1200 mm | 600 x 600 x 600 mm | Small labs, single user |
| BSC-3ft | 1000 x 750 x 1200 mm | 900 x 600 x 600 mm | Most common, 1-2 users [citation:4][citation:6] |
| BSC-4ft | 1200 x 750 x 1200 mm | 1100 x 600 x 600 mm | High throughput, 2 users |
| BSC-6ft | 1800 x 750 x 1200 mm | 1700 x 600 x 600 mm | Large labs, multiple users [citation:6] |
| Parameter | Specification | Custom Options |
|---|---|---|
| Construction Material | SS 304 / SS 316 [citation:1][citation:4] | Combination (SS inside, MS outside) [citation:1] |
| Filtration System | Pre-filter + HEPA (99.999% @ 0.3μm) | ULPA for ISO Class 3-4 |
| Airflow Velocity (Inflow) | 100 fpm (0.5 m/s) [citation:3] | 75-150 fpm adjustable |
| Airflow Velocity (Downflow) | 55 fpm (0.28 m/s) | Adjustable via motor speed controller |
| Noise Level | ≤ 65 dB [citation:4] | Low-noise blower option |
| Air Quality Standard | ISO Class 5 (ISO 14644-1) | ISO Class 3-4 available |
| Motor Blower | Dynamically balanced, 1/4 HP to 1 HP [citation:6] | IE2/IE3 efficient motors |
| Lighting | LED / Fluorescent with UV germicidal light [citation:6] | Timer-controlled UV |
| Front Window | 10mm toughened glass with sliding mechanism | Auto-counterbalance |
| Electrical Accessories | Digital pressure gauge, 5/15A switch socket, ON/OFF switches | |
| Class / Type | Size | Material | Price Range (INR) |
|---|---|---|---|
| Class I Biosafety Cabinet | 2-3 ft | MS / GI Powder Coated | ₹65,000 – ₹1,20,000 |
| Class II Type A2 | 3 ft | SS 304 | ₹1,10,000 – ₹1,60,000 |
| Class II Type A2 | 4 ft | SS 304 | ₹1,50,000 – ₹2,50,000 |
| Class II Type A2 | 6 ft | SS 304 | ₹2,00,000 – ₹3,50,000 |
| Class II Type B2 | 3-4 ft | SS 304 | ₹2,00,000 – ₹4,00,000 |
| Class III (Glove Box) | Custom | SS 316 | ₹3,00,000+ (Get Quote) |
Our bio safety cabinets are used in clinical laboratories, pharmaceutical industry, research institutions, healthcare facilities, food processing, and veterinary laboratories. For complete facility setup, explore our Cleanroom Turnkey Solution for pharmaceutical and healthcare applications.
As a trusted biosafety cabinet manufacturer in India, we offer in-house manufacturing at Mumbai, factory-direct pricing, complete customization, pan-India installation, annual certification services, and 1 year comprehensive warranty. We also manufacture Other Products including pass boxes, laminar work stations, and garment cabinets.
We manufacture complete biosafety cabinets at our Mumbai facility. No middlemen. No outsourcing. This means factory-direct pricing with complete quality control over every component.
Our cabinets are engineered specifically for Indian laboratory conditions including power fluctuations, high ambient temperatures, and dusty environments.
Unlike importers who sell fixed designs, we build to your exact specifications. Any size from 2ft to 6ft, any material SS 304 or SS 316, any filter grade H13 or H14 HEPA.
From design to installation to annual certification. Free site survey, professional installation by trained technicians, 1 year comprehensive warranty, and AMC options for hassle-free operation.
Years of experience in cleanroom equipment manufacturing. Hundreds of successful installations across India. Clients include pharmaceutical plants, hospitals, research labs, and diagnostic centers. ISO-certified manufacturing processes.
Because we manufacture in-house, our pricing beats imported alternatives by 20-30% while offering superior build quality. Request a quote to compare.
According to NSF/ANSI Standard 49, a biosafety cabinet MUST be certified annually. We provide complete certification services including HEPA filter integrity testing, airflow velocity verification, smoke pattern testing, and electrical safety checks. For cleanroom entry protocols, consider our Air Shower systems for personnel decontamination.
Daily Checks by User:
Monthly Checks:
Quarterly Checks:
Annual Professional Certification:
Free Installation & Commissioning
Our trained technicians install and certify every cabinet before handover.
Operator Training
We train your laboratory staff on proper usage, cleaning procedures, and daily checks.
Annual Maintenance Contract (AMC)
Spare Parts Availability
All parts available from our Mumbai warehouse for same-day dispatch:
Pan-India Service Network
Response within 48 hours in major cities including Mumbai, Delhi, Bengaluru, Chennai, Hyderabad, Pune, Ahmedabad, Kolkata, and Lucknow.
1 Year Comprehensive Warranty
Covers manufacturing defects, HEPA filter performance, motor blower, electrical components, and window mechanism. Extended warranty options available on request.
We specialize in manufacturing Bio Safety Cabinets with top-notch quality, which are designed specifically for ultimate indoor comfort.
Moreover, these cabinets are engineered to protect users working inside while keeping harmful germs at bay. By utilizing advanced technology, Bio Safety Cabinets ensure that the air remains clean and safe for conducting experiments.
As a result, this feature helps researchers work safely and confidently without any concerns about air quality. In addition, our cabinets provide peace of mind, allowing scientists to focus on their critical work without the distraction of potential hazards.
Peter Air Systems is a reliable leader in manufacturing biological safety cabinets, and our products are specifically designed to safeguard biological materials. Moreover, our cabinets are meticulously crafted to meet stringent safety standards. Consequently, they ensure a secure environment for managing hazardous substances in laboratories and medical settings.
In addition to our focus on safety, we are dedicated to maintaining the highest quality standards. Therefore, you can trust Peter Air Systems to deliver top-tier solutions for protecting both personnel and critical materials. Furthermore, our commitment to innovation ensures that we consistently provide reliable and effective products.
At Peter Air Systems, we prioritize quality and reliability in every cabinet we produce. Specifically, our bio safety cabinets are crafted with precision engineering and advanced technology to guarantee optimal performance and durability. Furthermore, each cabinet undergoes rigorous testing to ensure it meets industry standards for safety and functionality.
Moreover, our cabinets incorporate advanced technology to maintain a sterile atmosphere, preventing contamination and safeguarding the integrity of experiments. With efficient filtration systems and robust construction, they effectively control airflow and maintain cleanliness within laboratory settings. Our cabinets work seamlessly with Air Filters for maximum contamination control.
Recognizing the unique requirements of different industries and applications, we offer customizable solutions tailored to specific needs. Whether for research laboratories, medical facilities, or industrial settings, our bio safety cabinets provide tailored features and configurations to enhance operational safety and efficiency.
By choosing Peter Air Systems, laboratories and medical facilities worldwide trust our commitment to safety and excellence. Not only do our bio safety cabinets protect personnel and environments, but they also support the accurate and reliable outcomes essential for scientific research and medical procedures.
Ultimately, investing in Peter Air System’s bio safety cabinets means investing in the highest standards of safety and reliability for handling biological substances. Therefore, contact us today to discover how our tailored solutions can meet your specific safety needs and enhance operational efficiency.
Peter Air System specializes in designing customized biological safety cabinets tailored to meet specific needs. In addition, our cabinets are meticulously crafted to ensure the utmost safety and reliability in handling biological substances across various industries.
We understand that different industries and applications have unique requirements for handling hazardous materials. Therefore, our team at Peter Air System works closely with clients to design cabinets that precisely meet their safety and operational needs. For instance, whether for research laboratories, medical facilities, or industrial settings, our customized solutions offer advanced features and configurations to enhance safety and efficiency.
Moreover, each biological safety cabinet by Peter Air System undergoes rigorous quality checks and precision engineering. As a result, this ensures that every cabinet not only meets but exceeds industry standards for safety and functionality. Consequently, our commitment to quality means you can trust our cabinets to provide a secure environment for sensitive experiments and procedures.
Equipped with advanced technology and efficient filtration systems, our cabinets maintain a sterile environment. This, in turn, prevents contamination and supports the integrity of experiments and processes. Furthermore, the robust construction of our cabinets ensures durability and long-term reliability in demanding environments.
When you choose Peter Air System, you partner with a leader in biological safety solutions. Ultimately, our expertise in designing and manufacturing customized cabinets reflects our dedication to safety and excellence. Thus, you can trust us to deliver tailored solutions that enhance safety protocols and operational efficiency in your facility. Additionally, our commitment to innovation ensures that we continuously improve our products to meet evolving safety standards.
| CLASSIFICATION | |
|---|---|
| Class – l | protection for the user and surrounding environment, but no protection for the sample being tested |
| Class – ll | Protection for the user, environment and sample |
| Type A1/A2-re-circulate air back into the laboratory. A1 & A2 are similar but differentiated by the minimum average inflow velocity (FPM). | |
| Type B1/B2-B1 re-circulates a percentage of air into laboratory. B2 cabinets are entirely hard ducted. | |
| Class – lll | Also known as glove boxes, provides maximum protection; the enclosure is gas-tight, and all materials enter and leave through a dunk tank or double-door autoclave. |
| TECHNICAL PARAMETERS | |
|---|---|
| Construction | SS 304/SS 316 or combination of both |
| Filtration | 1st Stage Fine Filter with efficiency of 95% down to 5 micron |
| 2nd Stage HEPA Filter with efficiency of 99.999% down to 0.3 micron supply/exhaust position return air filter | |
| 3rd Stage-Fine Filter with efficiency of 95% down to 5 micron | |
| Motor Blower | Dynamically balanced with suitable ratings & suspension arrangement |
| LED Lights | |
| 5/15 Amp switch socket | |
| ON/OFF Switch | |
| Electrical Accessories | Digital Pressure Gauge |
| Air Quality | ISO Class 5 (ISO 14644-1: 1999) |
Your laboratory safety is our priority. Peter Air Systems delivers high-quality bio safety cabinets with genuine HEPA filters, robust SS 304 construction, and complete customization. From Class I to Class III, we have the right solution for your application.
With pan-India installation, annual certification, and reliable after-sales support, we ensure your cabinet remains compliant and performs optimally for years.
Call +91 8484 836 337 for a free quote or technical consultation.
A laminar flow hood provides only product protection by blowing HEPA-filtered air over the work surface. A biosafety cabinet provides three layers of protection: personnel (inward airflow barrier), product (HEPA-filtered downflow), and environment (HEPA-filtered exhaust). Never use a laminar flow hood for infectious agents as it does not protect the user.
